海产鱼类中异尖线虫酶消化检测技术的研究与应用     

许旭  黄维义  隋建新  林洪  鲜均章  励建荣  曹立民 《中国海洋大学学报(自然科学版)》2010,40(3)

以3种海鱼(牙鲆、狭鳕、鲱鱼)为样本,针对海产品中主要的致病性寄生虫-异尖线虫,开展了酶消化检测技术的研究.根据鱼肉消化前后干物质的质量变化设计了胃蛋白酶消化效率的计算方法,并按照鱼肉:消化液＝1:10 (g/mL)的反应比例,分别确定了酶水解鱼肉的最佳条件为:初始pH值为1.1,温度为37 ℃,酶活力为8 U/mL左右;消化后所得的虫体采用多重PCR方法替代传统的形态学观察进行种属鉴定,从而初步建立了基于酶水解-多重PCR的异尖线虫的酶消化检测体系.实际样本检测表明,该技术可以较为准确、快速地用于海产鱼类中简单异尖线虫(Anisakis simplex)和伪地新线虫(Pseudoterranova decipiens)的确证性检测.  相似文献   

Location,location, location: changes in the diversity of animal resources exploited by Tardiglacial humans in northern Spain     

Pablo Turrero  Eva García‐Vázquez  Miguel Arbizu  Gema E. Adán 《第四纪科学杂志》2010,25(2):214-221

Prey diversity of Homo sapiens in north Iberia exhibited shifts associated with climate warming after the Last Glacial Maximum, as deduced from animal remains obtained from Upper Palaeolithic caves in Asturias (northern Spain). Significant association of prey diversity and river proximity was found during adverse climate conditions, indicating opportunistic predation. A high increase of prey diversity was found when small animals (including fish) were taken into account in archaeological records, highlighting the importance of this type of prey in Palaeolithic diets. Copyright © 2009 John Wiley & Sons, Ltd.  相似文献   

Evidence of the restricted gene flow within a small spatial scale in the Japanese common intertidal goby Chaenogobius annularis     

Shotaro Hirase  Manami Kanno  Minoru Ikeda  Akihiro Kijima 《Marine Ecology》2012,33(4):481-489

Elucidating the scale of gene flow among populations is an important challenge for understanding the ecological dynamics and local adaptation of marine organisms. We assessed whether gene flow is restricted even at a small spatial scale in the Japanese common intertidal goby Chaenogobius annularis, using highly polymorphic DNA markers, involving the mitochondrial DNA (mtDNA) control region and 15 microsatellite DNA (msDNA), because past ecological studies have suggested low dispersal ability for rocky intertidal fishes. We found significant heterogeneities between four neighboring local populations by both mtDNA and msDNA analyses. In addition, no genetic heterogeneity was detected by either method across generations within a population; it was considered that such genetic differentiation is retained across generations and that the gene flow of this species is restricted to within a radius of a few kilometers. This is the first report showing a clear genetic subdivision in rocky intertidal fish.  相似文献   

Enhancing remote sensing research on global change to improve our understanding on Earth system processes     

JianCheng Shi 《中国科学:地球科学(英文版)》2014,57(10):2281-2282

正Global Change includes climate change and other environmental changes caused by the joint interaction among various layers of Earth. From the positive side, global change provides new opportunities to human and other living forms on Earth. In the meantime, it creates tremendous challenges and negative impact. At present, the negative impacts have reached all primary processes of the global ecosystem and every aspect of human society, especially causing degradation of the ecosystem. For instance, intensive deforestation causes decline of biodiversity; global warming causes sea level rise and increases  相似文献   

Composition and distribution of fish species collected during the fourth Chinese National Arctic Research Expedition in 2010     

LIN Longshan  LIAO Yunchih  ZHANG Jing  ZHENG Senlin  XIANG Peng  YU Xingguang  WU Risheng  SHAO Kwangtsao 《极地研究(英文版)》2012,23(2):116-127

There are awareness and concerns caused by the decreasing sea ice coverage around the Arctic and Antarctic due to effects of climate change. Emphasis in this study was on rapid changes in Arctic sea ice coverage and its impacts on the marine ecology during the fourth Chinese National Arctic Research Expedition in 2010. Our purpose was to establish a baseline of Arctic fish compositions, and consequent effects of climate change on the fish community and biogeography. Fish specimens were col- lected using a multinet middle-water trawl, French-type beam trawl, otter trawl, and triangular bottom trawl. In total, 36 tows were carried out along the shelf of the Bering Sea, Bering Strait, and Chukchi Sea in the Arctic Ocean. in total, 41 fish species belonging to 14 families in 7 orders were collected during the expedition. Among them, the Scorpaeniformes, including 17 species, accounted for almost one third of the total number （34.8%）, followed by 14 species of the Perciformes （27.0%）, 5 species of the Pleuronecti- formes （22.3%）, and 2 species of the Gadiformes （15.4%）. The top 6 most abundant species were Hippoglossoides robustus, Bore- gadus saida, Myoxocephalus scorpius, Lumpenus fabricii, Artediellus scaber, and Gymnoeanthus trieuspis. Abundant species var- ied according to the different fishing methods; numbers of families and species recorded did not differ with the various fishing methods; species and abundances decreased with depth and latitude; and species extending over their known geographic ranges were observed during the expedition. Station information, species list, and color photographs of all fishes are provided.  相似文献   

海洋生物多样性保护空间规划分区体系构建及其在泉州湾的应用     

颜利  王金坑  蒋金龙  傅世锋  戴娟娟  欧阳玉蓉 《台湾海峡》2012,31(2):238-245

基于海岸带综合管理的理念,通过海域生态环境敏感性评价与海域生物多样性保护重要性评价,综合利用地理相关分析法、空间叠置法等定性和定量分区相结合的方法进行海洋生物多样性保护空间规划分区划界,提出了海洋生物多样性保护空间规划分区体系,将规划区域划分为生物多样性重点保护区、生物多样性养护区和生物多样性保护重点监督区.以福建泉州湾为案例,应用海洋生物多样性保护空间规划分区体系,得出泉州湾生物多样性保护极重要地区是泉州湾河口湿地省级自然保护区,泉州湾生物多样性保护比较重要地区是晋江河口、洛阳江河口、晋江南岸地区及清源山.泉州湾海洋生物多样性保护空间规划分区方案共划定26个分区,包括生物多样性重点保护区3个,生物多样性养护区14个,生物多样性保护重点监督区9个.泉州湾的应用结果表明,本研究提出的海洋生物多样性保护空间规划分区体系具有一定的可行性和可操作性,可为泉州湾生物多样性保护管理提供重要科学依据.  相似文献   

外源化学物质对鱼类生长和GH/IGF-I轴的影响     

郑艳  汝少国 《中国海洋大学学报(自然科学版)》2012,(Z1):102-106

生长激素/胰岛素样生长因子-I轴(Growth hormone/insulin-like growth factor-I axis,GH/IGF-I轴)是调控鱼类生长的主要内分泌轴线,许多外源化学物质能够影响鱼类的生长,并且能够干扰GH/IGF-I轴。本文介绍了鱼类GH/IGF-I轴的组成、功能及其对鱼类生长的调控模式,并从GH/IGF-I轴角度探究外源化学物质影响鱼类生长的内分泌作用机制,展望了该领域的发展方向。  相似文献   

鱼类甲状腺轴对胚胎发育、仔鱼变态及性别分化的调控作用研究进展     

张晓娜  田华  汝少国 《中国海洋大学学报(自然科学版)》2012,(Z1):94-101

鱼类甲状腺轴由下丘脑、垂体、甲状腺组成,下丘脑通过分泌促甲状腺激素释放激素、促肾上腺皮质激素释放激素调节垂体促甲状腺激素的合成和分泌,促甲状腺激素促进甲状腺滤泡合成并释放甲状腺激素(主要为甲状腺素—T4),T4运输至肝脏等外周组织中,经过脱碘转化形成生物活性更强的三碘甲腺原氨酸(T3),T3主要通过同核受体结合发挥生物学功能。甲状腺激素对鱼类胚胎发育、仔鱼变态及性别分化等早期生长发育过程具有重要调控作用:甲状腺激素为鱼类胚胎发育期所必须,外加适量甲状腺激素能够提高胚胎或仔鱼存活率;仔鱼变态过程中,某些外部形态变化及相关组织分化依赖甲状腺激素的调控,牙鲆等仔鱼变态期体内甲状腺激素水平及其受体表达量会显著升高;性腺发育期,甲状腺激素对调控鱼类性别分化方向可能具有一定作用。  相似文献   

应用Excel 软件计算生物多样性指数   总被引：14，自引：0，他引：14  

孔凡洲  于仁成  徐子钧  周名江 《海洋科学》2012,36(4):57-62

生物多样性指数是生物群落研究中的重要参数,广泛应用于海洋生态系统生物多样性的评价和研究。作者利用微软的电子表格软件Excel中的数组函数、条件函数及其他函数,提供了一种快速、简便、可靠的同时计算Shannon、Simpson、Berger-Parker、Margalef和Pielou均匀度指数等8种常用生物多样性指数的方法,应用开发的Excel文件——Biodixcel.xlsx,使用者只需在此文件列出的表格中一次性录入所要计算的原始数据即可在此表格中同时得到上述几个指数的计算结果,本方法为群落生物多样性的计算提供了一种简单、实用的工具。  相似文献   

Tackling illegal, unregulated, and unreported trade towards Humphead wrasse (Cheilinus undulatus) recovery in Sabah, Malaysia     

Tun-Min Poh  Lucia M. Fanning 《Marine Policy》2012,36(3):696-702

The live reef food fish trade (LRFFT) has caused severe depletion of the Humphead wrasse (Cheilinus undulatus) populations globally, a species considered endangered by the International Union for the Conservation of Nature. In 2010, signatories to the Convention on International Trade in Endangered Species of Wild Fauna and Flora recognized that illegal, unregulated, and unreported (IUU) activities in the LRFFT were undermining the efforts to protect this species. Management recommendations have yet to be effectively implemented at national and local levels where the fish is harvested. This paper analyzes the threats to, and socio-economic impacts of, Humphead wrasse recovery in Sabah, Malaysia, and offers both short- and longer-term recommendations for the local recovery of the species. Key issues addressed centre around understanding the threats to recovery, specifically the role of IUU and the context-specific considerations, including the socio-economic aspects of the IUU trade in Sabah. The analysis confirms that deliberate management planning and action at multiple levels are required to prevent local-scale extinction. Lessons for other species and countries involved in the LRFFT suggest that to be effective, broad management frameworks must be tailored to local situations and must focus on gaining support and participation of fish harvesters if the recovery and long-term sustainability of both the Humphead wrasse and the LRFFT are to be achieved.  相似文献